Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives



Authors: Sadeghpour Heravi F, Zakrzewski M, Vickery K, G Armstrong D, Hu H
Publication: Journal of clinical medicine
Year: 2019


Diabetic foot ulcers (DFUs) and diabetic foot infections (DFIs) are associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. Diverse bacterial communities have been identified in DFUs/DFIs, playing a significant role in infection prognosis. However, due to the high heterogeneity of bacterial communities colonized in DFUs/DFIs, culture-based methods may not isolate all of the bacterial population or unexpected microorganisms. Recently, high sensitivity and specificity of DNA (metagenomics) and RNA (metatranscriptomics) technologies have addressed limitations of culture-based methods and have taken a step beyond bacterial identification. As a consequence, new advances obtained from DNA- and RNA-based techniques for bacterial identification can improve therapeutic approaches. This review evaluated the current state of play in aetiology of DFUs/DFIs on culture and molecular approaches, and discussed the impact of metagenomic and metatranscriptomic methods in bacterial identification approaches.

  • Author/s : Sadeghpour Heravi F, Zakrzewski M, Vickery K, G Armstrong D, Hu H
  • Publication : Journal of clinical medicine
  • Year : 2019
  • Volume : 8
  • Issue : 11
  • Article Keywords : 16S rRNA sequencing; culture; culturomics; diabetic foot infections; diabetic foot ulcers; metagenomics; metatranscriptomics; microbiology; microbiota